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mcd99Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40When coexpressed, mCD99 formed heterodimers with mCD99L2, as well as homodimers, and the heterodimers were localized more efficiently at the plasma membrane